Integrated Cell Biology (ICB)

Vakbeschrijving Integrated Cell Biology (ICB)
Collegejaar: 2016-2017
Studiegidsnummer: 4423ICBIOY (ICB)
  • Dr. C. Backendorf
  • Dr. M.C. van Eijk
Voertaal: Engels
Blackboard: Ja
EC: 2
Niveau: 500
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Actual course information
Up to date schedule information

Elective course in the MSc Chemistry and Life Science and Technology


Integrated Cell Biology is organized as a one-week course combining theoretical lectures on cell culture technologies in the morning with state-of-the-art practicals in the afternoon. The course will provide an efficient and integrated view on today’s possibilities to study, develop and exploit in vitro human cell cultures for biomedical research purposes. The course is limited to maximally 12 participants per session in order to promote efficient individual training.
Pre-registration via Blackboard is obligatory to participate.

Daily schedule

  • Day 1

Lecture 1 (9H00 – 11H00): ICB: Introduction to the experiments
Practicals (11H00 – 17H00 ): Initiation to sterile cell culture techniques, learn how to inspect cell cultures; initiate new cell cultures at various oxygen pressures (3 or 20%); transfection experiment using 3 different GFP-fusion constructs (chemical PEI transfection). Treat cells with nocodazole (mitotic poison) for later FACS analysis.

  • Day 2

Lecture 2 (9H00 – 11H00): Cell culture history and future
Practicals (11H00 – 17H00): Setup AIG (anchorage independent growth) experiment, passage cells for G418 selection, FACS (fluorescence activated cell sorting) & Flowing Software analysis.

  • Day 3

Lecture 3 (9H00 – 11H00): Culture of immortal (cancer) or normal human cells: pros and cons
Practicals (11H00 – 17H00): Analyze transfection experiment with fluorescent microscope. Fixation of cells on coverslips. IFA (immunofluorescence assay) staining of cells. Measure cell proliferation with Alamar Blue staining and Tecan analysis.

  • Day 4

Lecture 4 (9H00 – 11H00): Stem cells, organoids and regenerative medicine
Practicals (11H00 – 17H00: Fix and stain transfected cells for FACS analysis. Perform FACS analysis and analyze results with Flowing Software.

  • Day 5

Practicals (9H00 – 15H00): Inspect AIG experiment under microscope and quantify with Alamar Blue staining. Evaluate oxygen experiment. Compilation of data.
Final evaluation (15H00 – 17H00): Discussion and evaluation of experimental results.

Mode of instruction

Lectures, practicals, self-study (essay preparation)


Scientific publications and experimental protocols will be provided in pdf format via Blackboard. The following textbook is suggested as optional reading: Mummery, C., v.d. Stolpe, A., Roelen, B., Clevers, H. (2014) Stem Cells: Scientific Facts and Fiction. 2nd edition. Academic Press. ISBN: 9780124115514.

Prerequisites info

BSc in Life Science and Technology or MST/Chemistry


Pre-registration via Blackboard is obligatory to participate.


Students submit a short essay (1000 words) within the “Classical Cell Culture Experiments (CCCE)” theme and a practicals report written in article style (3000 words). Both submissions via Turnitin in Blackboard. The final mark will be composed as such: 40% essay + 40% Report + 20% Impression mark.


Claude Backendorf